Effect of abscisic acid (ABA) on the development regulation of somatic embryos from Coffea canephora P. var. Robusta

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Published: Jul 5, 2013
Keywords:
Coffea canephora, embryo culture, somatic cells, abscisic acid, totipotency

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María E. González
Instituto Nacional de Ciencias Agrícolas

Abstract

The somatic embryogenesis of plants, a perfect form of expression of cell totipotency, constitutes an important alternative for a large-scale production of desired individuals, asynchrony being one of the main causes limiting the successful development of this process. The present work was carried out with the objective of evaluating the effect of ABA on embryogenesis regulation in a liquid medium; thus, cell suspensions with a high embryogenic potential were selected in C-R and M-229 clones of Coffea canephora P. sp. var. Robusta from 28-day-formed calluses and subcultivated in MIE and MME media, where the following concentrations of ABA were studied: 0, 0.5, 1.0 and 2.0 mg.L-1. After appearing different developing stages of the somatic embryo, Petri dishes containing MDE semisolid medium were inoculated. The total number of somatic embryos (SE), SE number per developing stage and per each clone were evaluated. M-229 clone showed a better performance to form SE. There were significant differences among mean proportions of embryo production on behalf of 1.0 mg.L-1 ABA concentration, obtaining the biggest embryo number (1115). The lowest values were reached by ABA-lacking treatments and those with the tested concentration of 0.5 mg.L-1. The heart-shaped SE state reached values between 20.6 and 36.3, while the figures for the globular state did not surpass 24.8 embryos. The possibility of using ABA to diminish the degree of asynchrony in this crop was verified, as a result of its maturity effect on somatic embryos.

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How to Cite
González, M. E. (2013). Effect of abscisic acid (ABA) on the development regulation of somatic embryos from Coffea canephora P. var. Robusta. Cultivos Tropicales, 21(3), 33–37. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/772
Issue
Vol. 21 No. 3 (2000)
Section
Original Article

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