Alternative stainning technique to determine mycorrhizal colonization

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Published: Jun 10, 2015
Keywords:
arbuscular mycorrhiza, roots, colorants, health protection

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Yakelín Rodríguez Yon
Instituto Nacional de Ciencias Agrícolas (INCA)

Abstract

The root stainning technique is neccesary in the work with arbuscular mycorrhizal fungi (AMF), which constitute the base of several Biofertilizers, as the EcoMic® product, each time more used on agricultural practices at world level. That`s why the sample number evaluated at Mycorrhiza laboratories from diverse Research, Academic and even some productive Centers, increases every year. Those works require the application of such technique; nevertheless, it has the inconvenient to use highly toxic and carcinogenic reagents. The objective of the present work was to substitute those reagents by others non harmful to human health and environment. This is the case of Trypan blue that was substituted by the washable pen ink Parker QuinK. Moreover, Lactic acid and Glycerin were eliminated from the protocol. Results showed the pertinence of such modifications and revealed the usefulness and high quality of mentioned ink, which successfully stains all fungal structures allowing a clear visualization during long time.

Article Details

How to Cite
Rodríguez Yon, Y. (2015). Alternative stainning technique to determine mycorrhizal colonization. Cultivos Tropicales, 36(2), 18–21. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/961
Issue
Vol. 36 No. 2 (2015)
Section
Original Article

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