Determination of genetic stability in four in vitro preserved potato species from Cuban germplasm bank
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Abstract
studied for ten years, with the aim of determining if they kept
their genetic stability when compared to those from the base
collection remaining in the field. Species of both origins were
morphologically described; isozymatic and molecular methods
were used to evaluate if changes took place in the band patterns
of in vitro-preserved species. The isozymatic systems,
peroxidases, esterases, polyphenol oxidases and carbon
anhydrase, and the AFLP molecular marker were employed.
The four preserved species used were: Solanum schruteri Buk,
Solanum dolichostigma Buk, Solanum jamesii Jorr and
Solanum tuberosum L. Seedlings were preserved in a slow
growth medium and sub-cultivated twice a year. The qualitative
characters, yield and its components were evaluated, to determine
if there were phenotypical changes among the species studied.
Five primer combinations were used in the AFLP analysis. A t
test of media comparison was made to yield and its components,
to calculate if there were differences among accessions. There
were not any remarkable phenotypical changes for the
qualitative characters, yield and its components. Isozymatic
systems did not detect changes in the band patterns, while
AFLP analysis showed notable differences concerning band
number; thus, it is concluded that genetic stability is not
maintained in the different species studied during the
preservation period evaluated.
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