In vitro propagation of Moringa oleifera Lam. cultivars
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Abstract
a high level of vitamins, minerals and proteins of interest
for the food and medical industries. This species can be
propagated by seeds or cuttings, methods that not suitable
to introduce new cultivars. The aim of this research was
to establish an in vitro propagation protocol of different
cultivars of Moringa oleifera Lam. To disinfect seeds three
different disinfection times were evaluated five, seven and
nine minutes in 10 gL-1 sodium hipoclorite. Two methods for
in vitro micropropagation were used: conventional system
(semi-solid culture medium) and temporary immersion
bioreactor. In the in vitro rooting phase, four concentrations
of the naphthalene acetic acid and Indole butyric acid
auxins (0; 2,5; 5,0 and 7,5 μmol L-
1) were evaluated and
also the kind of explant. The survival rates of the sprout
during the acclimatization phase was evaluated in relation
to their origin in the rooting phase. Besides the effect of the
genotype on in vitro establishment and multiplication was
determined. The disinfection of the Supergenius cultivar
was better at seven minutes of treatment with sodium
hipoclorite, with 54 % of germination. The Temporary
Immersion Bioreactor increased the sprouts morphological
quality and multiplication coefficient from 6,2 until 16,1 of
the Supergenius cultivar. Rooting was successful without
auxin and acclimatization survival was around 85 % at
35 days for shoots rooted without growth regulators,
regardless of explant origin. It was shown that the genotype
influenced the establishment and multiplication of explants.
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