Obtaining a new rice seed disinfection method
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Abstract
the main issues when identifying endophytic bacteria as well
as with microorganism-inoculating trials. Disinfection by
chemical methods has been a commonly used strategy with
this goal. However, it is very hard to fully disinfect rice seeds,
due to its particular characteristics (rough and velvety surface),
so that most disinfection methods described by literature are
often useless. Therefore, this investigation was aimed at
searching an efficient rice seed disinfection methodology.
Then, seeds from cv. J-104 were used and four disinfection
protocols were assessed by employing different disinfecting
solutions (chloramine T, calcium hypochlorite and alcohol,
among others), exposure times and solution combination.
Results showed that by disinfection protocol 4, using calcium
hypochlorite in two plant growth stages, the best germination
and disinfection rates were achieved, obtaining an efficient
disinfection method, which could be applied to plantmicroorganism
interaction studies, from the practical point of
view.
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