The evaluation of different substrates for vitroplant and microbulblet acclimatization of garlic (Allium sativum L.)
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Abstract
asexually reproduced; therefore, the application of different
biotechnological techniques and its further adaptation to
environmental conditions are very important for its cultivation
in Cuba. Therefore, this study was carried out at �??Liliana
Dimitrova�?� Horticultural Research Institute, with the objective
to evaluate garlic vitroplant and microbulblet development in
the adaptation phase, to determine the best substrate for
microbulblet development in the acclimatization phase and to
evaluate genotype effects in this substrate. Then, vitroplants
from the third multiplication subculture and in vitro-induced
microbulblets were taken with 75 g.L-1 sucrose from clones
�??Criollo-3�??, �??Criollo-6�??, �??Criollo-9�??, �??Martínez�?? and �??Vietnamita�??;
they were planted in a substrate made up by 50 % filter cake
and 50 % soil. After that, microbulblets were planted in different
substrates. Percentages of survival and rooting, plant height
and leaf number/plant were evaluated in each case. A
Randomized block design with four replicates was used and
data were processed through a one-way classification variance
analysis. Consequently, microbulblets are better adapted than
vitroplants to these environmental conditions and reach a
higher percentage of survival. The combination of zeolite (25 %)
and filter cake (75 %) as substrate enabled microbulblet plants
to reach the highest suvival and rooting in every garlic
genotype studied.
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