Screening of ex situ preserved tomato (Solanum L. section Lycopersicon) germplasm in Cuba by using in vitro tests to predict high temperature responses
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Abstract
of evaluating thermotolerance at tissue level in a representative
sample of ex situ preserved tomato (Solanum L. section
Lycopersicon subsection Lycopersicon) germplasm in Cuba,
besides identifying the most tolerant accessions to be used in
plant breeding programs. Thus, cell viability was measured by
reducing tetrazolium salt to formazan through dehydrogenase
enzyme action in 122 tomato accessions, which were classified
for their thermotolerance, taking into account three well known
controls: Nagcarlang, Amalia and AN-104-1. Concerning the
ex situ preserved germplasm in Cuba, there are thermotolerant
accessions, mainly from S. lycopersicum var. Cerasiforme and
S. pimpinellifollium, that can be employed in plant breeding
programs, to obtain good-behaving varieties and hybrids
under these conditions.
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