Residents rhizobia in the rhizosphere of rice plants (Oryza sativa L.) cultivar INCA LP-5

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Ionel Hernández Forte
María Caridad Nápoles García

Abstract

The objective of this work was to isolate
rhizobia from the rhizosphere of rice plants cultivar
INCA LP-5. Rhizobia isolation from rhizospheric soil
and rhizoplane of rice plants growing in Petroferric
Ferruginous Nodular Gley soil was conducted.
The possible taxonomic distribution of the isolates
was determinated throught the study of their morphocultural
and physiological characteristics. In vitro
nodulation assays were also performed in Macroptilium
atropurpureum (DC.) Urb. (siratro). Fourteen isolates
showed similar cultural and morphological characteristics
to rhizobia, of which 28,57 % were obtained from the
rhizospheric soil and 71,43 % from rhizoplane of rice
plants. All isolates were negative to cetolactase test and
produced acid. An isolated showed moderate growth
on the culture medium while the rest grew faster.
Eleven rhizospherics isolated from rice plants produced
effective nodules in siratro plants. A possible member
of the Mesorhizobium genera and ten possible members
of Rhizobiaceae family were identified. Four isolates
showed a high effectiveness of the nodulation process
in siratro plants and one of them significantly increased
the root dry mass. The results obtained in this study
provide the first evidence in Cuba where interaction of
rhizobia-rice is developed.

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How to Cite
Hernández Forte, I., & Nápoles García, M. C. (2017). Residents rhizobia in the rhizosphere of rice plants (Oryza sativa L.) cultivar INCA LP-5. Cultivos Tropicales, 38(1), 39–49. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1333
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Original Article