Effect of the somatic embryos morphology on plant regeneration of soybean (Glycine max L. Merrill)
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Abstract
The established protocols for plant regeneration via somatic embryogenesis in soybean (Glycine max L. Merrill) are specific to a small number of cultivar, requiring new studies to provide more efficient and reproducible protocols in a large number of genotypes. This work had as objective to determine the effect of the morphology type of somatic embryos on the germination and plant conversion in soybean cultivar ʻIncasoy-27ʼ. Mature somatic embryos were used with different morphologies types that were placed in germination medium. At 30 days the somatic embryos number with complete or partial germination by morphology type was quantified. In the conversion phase it was determined to the 10 days of cultivation the survival percentage of the regenerated plants and it was carried out a morphological characterization of the regenerated plants in comparison with the obtained plants of seeds. It was achieved at 74,38 % of somatic embryos germinated with normal and abnormal morphology. The percentage of survival of regenerated plants reached 70,0 % efficiency. The progeny obtained from the regenerated plants of somatic embryos were morphologically similar to the control plants. The results from this research show that it is possible to allow regeneration of whole plants through somatic embryogenesis in soybean Cuban cultivar ʻIncasoy-27ʼ.
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