Germination and development of Rhizoglomus sp. propagules in vitro
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Abstract
The physiological studies related to the mycorrhizal symbiosis have made difficult due to the condition of the symbionts that are characterized by the fungi that intervene in the association, which, up to now, can’t grow in axenic conditions. However, in vitro root cultivation techniques, as a simplified system for the establishment of the arbuscular mycorrhizal symbiosis, have been widely used and they are very important in several investigations. The aim of this study was to obtain disinfected spores of Rhizoglomus sp. (INCAM-11) from soil, which express high percentages of germination and low levels of contamination on in vitro conditions and determine the growth period of the germination hyphae. INCAM-11 propagules were disinfected and their percentages of germination and contamination, as well as the germinative tube length were evaluated. Results show that the immersion time of spores in antibiotic solution during 24 hours, before its inoculation in the Modified Strulla and Romand (MSR) medium, can reduces the contamination percentage and the moment of germination. In the other hand, the hyphae germination spreads in the culture medium during 30 days approximately, before stopping their growth, contributing to the later establishment of the association in the transformed roots.
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