Acclimatization of Lisianthus (Eustoma grandiflorum (Raf.) Shinners) seedlings ‘Mariachi blue’ cultivar with an oligogalacturonide
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Abstract
Oligogalacturonides are substances that regulate the growth and plant development and stimulate defensive responses; however, there is no information on the effects that these compounds exert when in vitro seedlings of Lisianthus (Eustoma grandiflorum (Raf.) Shinners) are transferred to ex vitro conditions. Therefore, an experiment was carried out with the objective of evaluating the oligogalacturonide influence of pectic origin (mOLG) in Lisianthus seedlings of cultivar ‘Mariachi blue’ during the first acclimatization phase. The treatments consisted on seedling foliar spraying at transplantation time and 15 days after it, at a rate of 2 mgL-1 of IAA; 1, 5 and 10 mgL-1 of mOLG and a control treatment was used in which no foliar spraying was performed. Morphological variables were evaluated: height and number of leaves per plants and physiological variables: fresh and plant dry mass, relative water content (RWC) and photosynthesis. The experiment was carried out in a completely randomized design with three replications. The data were processed using a simple ANOVA and the means between the treatments were processed using the Tukey test (p£0.05). Plants sprayed with mOLG (1 mgL-1), reached the highest height [9.37 cm], the number of leaves ranged between 9-10; and those that were treated with mOLG (10 mgL-1), presented higher fresh mass; However, the plants treated with mOLG (1 mgL-1), reached higher dry mass (0.209845 g), CRA (90.75 %) and photosynthesis
(12.873 μmol CO2 m-2s-1). There is potential for the cultivation of this cut flower in Cuba.
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