Isolation and characterization of rizobia strains from chickpea nodules (Cicer arietinum L.)
Article Sidebar
Main Article Content
Abstract
Chickpea (Cicer arietinum L.) is a leguminous of wide acceptance in Cuba; it constitutes tolerant specie to adverse environmental conditions, besides their nutritional contribution. Rizobia, which habit inside the chickpea nodules, contribute with benefits to the plant that is why they could be used to elevate their yields and to extend the crop in the country. The objective of this study was to obtain isolates of possible rizobia associated with different chickpea varieties and soil types in Cuba, as well as to characterize them in terms of their tolerance to different pH and temperature values. From seven chickpea varieties sowed in four soil types, 63 bacterial isolates were purified. From them, 11 isolates as possible rizobios by their morphophysiologic and biochemical characteristics were selected. Three of these isolated stood out by tolerate acids and basic pH values and high temperatures, so they could be promissory to constitute active ingredients of new inoculants for this vegetable specie.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.
References
Apáez Barrios M, Escalante Estrada JAS, Apáez Barrios P, Álvarez Hernandez JC, Apáez Barrios M, Escalante Estrada JAS, et al. Producción, crecimiento y calidad nutrimental del garbanzo en función del nitrógeno y fósforo. Revista mexicana de Ciencias agrícolas [Internet]. 2020 [cited 2024 Feb 1];11(6):1273–84. doi:10.29312/remexca.v11i6.2226
Zhang J, Peng S, Shang Y, Brunel B, Li S, Zhao Y, et al. Genomic diversity of chickpea-nodulating rhizobia in Ningxia (north central China) and gene flow within symbiotic Mesorhizobium muleiense populations. Systematic and Applied Microbiology [Internet]. 2020 [cited 2024 Feb 1];43(4):126089. doi:10.1016/j.syapm.2020.126089
Rocha JFL de la, Sánchez DMS, Cortez JAJ. Efecto de la fertilización nitrogenada y fechas de siembra en el cultivo de garbanzo (Cicer Arietinum L.) en Tehuacán, Puebla, México (Original). Roca. Revista científico-educacional de la provincia Granma [Internet]. 2019 [cited 2024 Feb 1];15(3):25–34. Available from: https://revistas.udg.co.cu/index.php/roca/article/view/925
Vincent JM. A manual for the practical study of root-nodule bacteria [Internet]. . International Biological Programme. London; 1970. 164 p. Available from: https://www.amazon.com/manual-practical-root-nodule-bacteria-handbook/dp/0632064102
Madigan M, Bender K, Buckley D, Sattley W, Stahl D. Brock Biology of Microorganisms [Internet]. NY, U.S.A; 2017. 1056 p. Available from: https://www.amazon.com/Brock-Biology-Microorganisms-Michael-Madigan/dp/0134261925
Thies JE, Singleton PW, Bohlool BB. Modeling symbiotic performance of introduced rhizobia in the field by use of indices of indigenous population size and nitrogen status of the soil. Applied and Environmental Microbiology [Internet]. 1991;57(1):29–37. doi:10.1128/aem.57.1.29-37.1991
Harrigan WF, McCance ME. Métodos de laboratorio en microbiología [Internet]. España: Academia; 1968. 426 p. Available from: https://biblioteca.epn.edu.ec/cgi-bin/koha/opac-detail.pl?biblionumber=39933
Ortega-García M, Ríos-Rocafull Y, Zelaya-Molina L, Lara-Aguilera J, Arteaga-Garibay R, Nápoles-García MC. Rhizobium pusense associated to chickpea (Cicer arietinum L.), in Cuba. Agronomía Mesoamericana [Internet]. 2024 [cited 2024 Feb 6];55876–55876. doi:10.15517/am.2024.55876
Aroche EJA, Reina EM, Llanes JM. Diagnóstico inicial de la evolución de un suelo degradado. Avances [Internet]. 2019 [cited 2024 Feb 6];21(1). Available from: https://www.redalyc.org/journal/6378/637869112010/html/
Velázquez E, García-Fraile P, Ramírez-Bahena M-H, Rivas R, Martínez-Molina E. Current Status of the Taxonomy of Bacteria Able to Establish Nitrogen-Fixing Legume Symbiosis. In: Zaidi A, Khan MS, Musarrat J, editors. Microbes for Legume Improvement [Internet]. Cham: Springer International Publishing; 2017 [cited 2024 Feb 6]. p. 1–43. doi:10.1007/978-3-319-59174-2_1
Egamberdieva D, Wirth SJ, Shurigin VV, Hashem A, Abd_Allah EF. Endophytic Bacteria Improve Plant Growth, Symbiotic Performance of Chickpea (Cicer arietinum L.) and Induce Suppression of Root Rot Caused by Fusarium solani under Salt Stress. Frontiers in Microbiology [Internet]. 2017 [cited 2024 Feb 6];8:1887. Available from: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2017.01887
Sahgal M, Jaggi V. Rhizobia: Culture Collections, Identification, and Methods of Preservation. In: Sharma SK, Varma A, editors. Microbial Resource Conservation: Conventional to Modern Approaches [Internet]. Cham: Springer International Publishing; 2018 [cited 2024 Feb 6]. p. 175–97. (Soil Biology). doi:10.1007/978-3-319-96971-8_6
American Society for Microbiology., Microbiology AS for, Bergey DH, Breed RS. Bergey’s manual of determinative bacteriology [Internet]. 7th ed. Baltimore: Williams & Wilkins Co; 1957. 1130 p. doi:10.5962/bhl.title.10728
Singha B, Mazumder PB, Pandey P. Characterization of Plant Growth Promoting Rhizobia from Root Nodule of Two Legume Species Cultivated in Assam, India. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences [Internet]. 2018 [cited 2024 Feb 6];88(3):1007–16. doi:10.1007/s40011-016-0836-6
Ojuederie OB, Olanrewaju OS, Babalola OO. Plant growth promoting rhizobacterial mitigation of drought stress in crop plants: Implications for Sustainable Agriculture. Agronomy [Internet]. 2019 [cited 2024 Feb 6];9(11):712. doi:10.3390/agronomy9110712
Andrews M, Andrews ME. Specificity in Legume-Rhizobia Symbioses. International Journal of Molecular Sciences [Internet]. 2017;18(4):705. doi:10.3390/ijms18040705
Hussain HA, Hussain S, Khaliq A, Ashraf U, Anjum SA, Men S, et al. Chilling and Drought Stresses in Crop Plants: Implications, Cross Talk, and Potential Management Opportunities. Frontiers in Plant Science [Internet]. 2018 [cited 2024 Feb 6];9:1–21. Available from: https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00393
Padilla EG, Ruiz-Díez B, Fajardo S, Eichler-Loebermann B, Samson R, Damme PV, et al. Caracterización de rizobios aislados de nódulos de frijol caupí, en suelos salinos de Cuba. Cultivos Tropicales [Internet]. 2017 [cited 2024 Feb 6];38(4):39–49. Available from: https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1401
Rodiño AP, Riveiro M, De Ron AM. Implications of the Symbiotic Nitrogen Fixation in Common Bean under Seasonal Water Stress. Agronomy [Internet]. 2021 [cited 2024 Feb 6];11(1):70. doi:10.3390/agronomy11010070
Dhull S, Gera R, Sheoran HS, Kakar R. Phosphate Solubilization Activity of Rhizobial Strains Isolated From Root Nodule of Cluster Bean Plant Native to Indian Soils. International Journal of Current Microbiology and Applied Sciences [Internet]. 2018 [cited 2024 Feb 6];7(04):255–66. doi:10.20546/ijcmas.2018.704.029
Velazquez E, Carro L, Flores-Felix JD, Menendez E, Ramirez-Bahena MH, Peix A. Bacteria-inducing legume nodules involved in the improvement of plant growth, health and nutrition. 2019 [cited 2024 Feb 6]; doi:10.1007/978-981-13-8495-0_4
Lebrazi S, Fadil M, Chraibi M, Fikri-Benbrahim K. Screening and optimization of indole-3-acetic acid production by Rhizobium sp. strain using response surface methodology. Journal of Genetic Engineering and Biotechnology [Internet]. 2020 [cited 2024 Feb 6];18(1):21. doi:10.1186/s43141-020-00035-9