Obtaining transconjugant strains for bacteria-rice interaction studies
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Abstract
The objective of this work was to obtain transconjugant strains for the study of plant-bacteria interaction. The obtaining of transconjugant bacteria with the plasmid pHC60 was carry out by triparental mating with two Escherichia coli strains and two strains isolated from Cuban rice cultivars. Furthermore, an inoculation assay with rice plants cv. INCA LP-5 and transconjugant bacteria was carry out and confocal fluorescence microscopy were used to locate the bacteria in plant tissues. Two transconjugant strains containing gfp gene were obtained, since they produced fluorescent colonies in ultraviolet light, in culture medium with tetracycline and 5-nitrofurantoin. The multiplication dynamics of wild type and transconjugant strains don’t showed differences in the end of bacteria growth in the medium. Two transconjugant strains were showed colonizing rice root, one of them as a putative endophyte.
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