Chemical characterization and evaluation of the biological activity of Spirulina extracts
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Abstract
The increase in the yield and productivity of crops, without affecting the environment, is one of the challenges that farmers experience today. The use of Spirulina (Arthrospira platensis) extracts as a plant biostimulant is one of the most viable options to use for these purposes. The objective of this work was to obtain, characterize and evaluate the biological activity of some alcoholic extracts of Spirulina. For this, extracts were prepared from Spirulina powder using ethanol as solvent in two concentrations (70 and 90 %), two mass:solvent ratios (1:20 and 1:10) and two maceration times (10 and 21 days) and the content of proteins, phenols and flavonoids of each was determined by spectrophotometric determinations. Biological activity was evaluated using a rice seed germination assay from seed imbibition for 24 h and the germination process was carried out in Petri dishes with distilled water for seven days. The results obtained from the chemical characterization will allow to adapt the extraction parameters depending on the components that are to be favored and the physiological effect that is to be achieved, since the concentration of proteins, phenols and flavonoids in the extracts significantly influenced the percentage end of germination of rice seeds.
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