Effect of Trichoderma harzianum and Pseudomonas fluorescens on the control of anthracnose in mango fruit (Mangifera indica L.) during post-harvest
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Abstract
The objective of the study was to evaluate the effect of Trichoderma harzianum and Pseudomonas fluorescens on the control of anthracnose in post-harvest mango fruit. An experimental study was conducted on Tommy Atkins mangoes using beneficial microorganisms at different concentrations as treatments. A completely randomized design was established with six treatments and ten replicates; the microorganisms were applied by spraying for four months. The incidence, severity, and total soluble solids (°Brix) were evaluated, and the data were analyzed using ANOVA and Tukey (α = 0.05). Molecular identification confirmed the high purity of T. harzianum (KF624792), P. fluorescens (MG977684), and the pathogen C. gloeosporioides (MH700455). The results showed that all treatments with beneficial microorganisms achieved 0% incidence, while the control treatment had a 10 % incidence with visible necrotic lesions. In addition, the consortium of both microorganisms (T5) recorded the highest concentration of soluble solids (15 °Brix), showing an improvement in fruit quality. The use of biological control microorganisms, both individually and in consortium, strengthened the fruit's defense mechanisms against C. gloeosporioides, providing post-harvest protection, increasing °Brix, and extending the shelf life of mangoes.
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