Effect of systemic fungicide on the presymbiotic growth of Rhizophagus irregularis (INCAM 11), in vitro
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Abstract
Fungicides are widely used in current culture systems to control or eliminate fungal plant pathogens. However, these chemicals can affect indigenous soil microorganisms, including those that promote plant growth, such as arbuscular mycorrhizal fungi (AMF). Considering the above exposed, the present study set out to determine the effect of different concentrations of the systemic fungicide Previcur energy 84 sl on the presymbiotic stage of Rhizophagus irregularis (INCAM 11), under in vitro culture conditions. Modified Strullu and Romand culture medium (SRM) was employed to evaluate percentage of germination, growth of germinating tube, as well as its percentages of increase and decrease, when studying four concentrations of the Previcur Fungicide (0,1; 1; 10 and 100 mg L-1). At 10 mg -1 a stimulating effect on germination and on the growth of the germ tube of the fungus. The present work constitutes the first evidence on the effect of a systemic fungicide in the presimbiotic stage of mycorrhizal fungus on in vitro conditions reported in Cuba.
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